StudiesThe Tools and Techniques of Protein Science: Protein Separation The Science Advisory Board's recent study, "Protein Separation" provides a number of interesting insights into the most current areas of investigation in protein and proteomic research. It is the third in an eight-part series of studies entitled, "The Tools & Techniques of Protein Science Catalyzing the Future of Proteomics." More than 500 scientists engaged in protein research took part in this study. Results indicate that the most preferred separation technique is one-dimensional SDS-polyacrylamide gel electrophoresis (PAGE) under reducing conditions. This was followed by one-dimensional SDS-PAGE under nonreducing and then one-dimensional SDS-PAGE under non-denaturing conditions. Respondents primarily use one-dimensional isoelectric focusing, one-dimensional SDS-PAGE or 2DGE for protein analysis or characterization experiments. 2DGE is also widely used by respondents for protein identification. Non-2DGE separation techniques are primarily used for protein purification. Most respondents who use multiple separation techniques employ either single or multiple, tandem liquid chromatography approaches with or without subsequent mass spectrometry. Over the next twelve months, usage of two cutting-edge approaches to separation technology -- capillary gel electrophoresis and microfluidics "lab-on-a-chip" -- in conjunction with mass spectrometry will significantly increase. However, over three-quarters of these scientists do not plan to use an integrated proteomics system that includes instrumentation specifically for protein separation. The Science Advisory Board is an online panel of more than 6,200 scientists, physicians and other life science and medical professionals from 62 countries. By convening electronically, Science Advisory Board members participate in online studies to voice their opinions on issues that directly affect the evolution and development of the tools and techniques of their professions. If you are interested in participating in other studies on the tools and techniques of your profession, please register for our Research Panel, or contact Molly Scott, Membership Coordinator, at m.scott@scienceboard.net for membership information and study details. Your identity and personal information will be held in the strictest confidence, and you will receive compensation for any studies in which you choose to participate. [ View Current & Future Studies ] [ View Past Studies ] |
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