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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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Enzymology and Protein Assays
10/8/2003 Kinase end-labeling of DNA  
10/17/2003 Conjugation of Antibody to HRP
10/17/2003 In vitro Sphingomyelinase Assay
10/17/2003 Radioiodination of protein in solution
10/17/2003 Radioiodination of cell surface proteins
10/17/2003 Annexin V Staining Protocol
10/18/2003 Tritiated thymidiene Uptake assay and MTT assay
10/18/2003 Measurement of Cell Adhesion Under Static Conditions
10/18/2003 The Under-Agarose Migration Assay
10/18/2003 Chloramphenicol Acetyl Transferase (CAT) Assay using n-Butryl Coenzyme A
10/19/2003 Determination of the Molecular Weight of a DNA Binding Protein
10/20/2003 In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells
10/20/2003 Polymerase III In Vitro Transcription
10/27/2003 In-Gel Kinase Assay
10/27/2003 Histone H1 Kinase Activity Assay
10/27/2003 FAK Autophosphorylation Assay
10/29/2003 Quick Virus/Viron Purification Protocol
11/7/2003 Use of hexosaminidase in a cytotocicity assay
11/18/2003 Yeast Beta-gal Assay
11/18/2003 Purification of the NSP1 RD Protein
11/18/2003 Actin Capture Assay
11/18/2003 MAKING YEAST EXTRACTS for WESTERNS
11/18/2003 Yeast Whole Cell Extracts
11/18/2003 Yeast Cell Lysates
11/18/2003 Yeast Rapid Protein Prep
11/18/2003 Yeast Glass Bead Prep
11/18/2003 Yeast Sucrose Gradient Protocol
11/18/2003 Yeast Nuclear Extract
11/19/2003 Sephadex G-50 spun column purification
11/21/2003 Analysis of soluble/insoluble partitioning of E. coli overexpressed proteins
11/21/2003 Growth of Prototrophic E. coli Strains in D2O Minimal Salts Media
11/21/2003 Buffer Exchange Procedure for the Preparation of NMR Samples
11/21/2003 Procedure for Ion (DEAE) Exchange Chromatography for ecT bR2 Purification
11/21/2003 Growth, Purification, and Refolding of TGF-b3 (for 3L)
12/28/2003 A generic Phosphatase and pyrophosphatase assay
6/11/2004 Detection of Phospho-Hsp27 in THP.1 cells by Bioplex Assay
10/31/2006 Quantitate Protein Immobilized on a Solid Support Using Coomassie Blue
11/17/2006 Radiochemical and fluorometric assays for plasmalogen-selective phospholipase A2
1/18/2007 Gelatinase in situ zymography
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