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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.

 
DNA Sequencing
10/8/2003 5'-RACE  
10/19/2003 DNase I Footprinting
10/20/2003 S1 Nuclease Mapping Using Single-Stranded Radiolabeled Oligonucleotide Probes
10/21/2003 Labeling Oligonucleotides Using T4 Polynucleotide Kinase
10/21/2003 Random Primed Labeling of DNA
11/13/2003 A rapid method for generating gene deletions in Aspergillus fumigatus
11/13/2003 Method for assaying gliotoxin production in Aspergillus fumigatus
11/18/2003 Replication timing by comparative hybridization
11/18/2003 Replication timing by density transfer
11/18/2003 Replication timing using transient hemimethylation
11/18/2003 In vitro Mutagenesis with dut ung single stranded DNA
11/18/2003 Measurement of DNA concentration using PanVera fluorescence assay
11/18/2003 DNA Sequencing Sequencing reaction
11/18/2003 DNA sequencing gels
11/20/2003 Bst-catalyzed radiolabeled DNA sequencing For single-stranded DNA sequencing
11/20/2003 Radiolabeled sequencing gel preparation, loading, and electrophoresis
11/20/2003 Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
11/20/2003 Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
11/20/2003 For Double-stranded reactions: Sequenase[TM] catalyzed sequencing with dye-labeled terminators
5/15/2004 DM1 Alu Polymorphism
5/15/2004 ACADM TaqI Polymorphism
5/15/2004 D10S94 PvuII Polymorphism
5/15/2004 RBP3 MspI Polymorphism
5/15/2004 DRD2 TaqI "B" Polymorphism
5/15/2004 RBP3 BglII Polymorphism
5/15/2004 DRD2 TaqI "A" Polymorphism
5/15/2004 NGFR TaqI(a) Polymorphism
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