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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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Protein Electrophoresis
10/17/2003 Silver Staining SDS PAGE Gels
10/17/2003 TCA protein precipitation
10/17/2003 Tricine-SDS-PAGE
10/18/2003 Detection of IkB by Western Blotting
10/19/2003 2D Gel Assay for RNA-Binding Proteins
10/19/2003 Stripping and Reusing Western Blots
10/29/2003 Preparation of Yeast Cell Lysate for Western Blotting
12/4/2003 protein extraction from plant tissues for isoelectric focusing: SDS extraction followed by acetone precipitation
12/4/2003 protein extraction for isoelectric focusing Phenol extraction followed by methanolic ammonium acetate precipitation
12/4/2003 Immunoprecipitation
9/27/2004 Acid Urea Triton (AUT) PAGE of Histones
10/15/2004 Gelatin zymography
6/14/2005 Alternative TCA protein precipitation
3/6/2006 Preparation of whole cell lycates from mammalian cells
6/7/2006 Extraction of membrane proteins
6/16/2006 30 min protocol for silver staining of protein gels.
8/24/2006 Two-Dimensional Gel Electrophoresis - From Start to Finish
3/22/2007 Coomassie Brilliant Blue (CBB) staining
3/26/2008 Gelatin-substrate zymography
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